Evaluasi Suplementasi Indigofera zollingeriana Sebagai Sumber Green Protein concentrate Terhadap Produksi Gas Metan, Amonia dan Sintesis Protein Mikroba Rumen
Abstract
The use of protein with low-cost, high quality, low methane, and ammonia emissions are a prerequisite as a protein source in ruminant. However, the European Commission has prohibited protein derived from fish meals for ruminant feeds. So encouraging efforts to explore the other protein sources to be most important. Most of the high protein legumes grow in tropical areas such as Indonesia and have the potential as an alternative protein source in ruminant feed, including Indigofera zollingeriana (25-27% protein content). But many browse legumes with high protein are a heterogeneous group of plants, with variable secondary metabolic content and rumen degradable protein. The aim of this experiment was to evaluate the characteristics fermentation of IZ as green protein supplement on in vitro methane, ammonia and microbial protein production. The experiment was a completely randomized design with four different level supplementation of Indigofera zollengeriana (IZ) as green protein concentrate and five replications. The treatment diets were R0; basal diet (60% forage + 40% concentrate) + 0% IZ, R1; R0 + 10% IZ, R2; R0 + 20% IZ, and R3; R0 + 30% IZ. The experiment result showed that supplemenatation of IZ was significant effects (P<0.05) to increase total gas, ammonia (N-NH3), total volatile fatty acid (TVFA), and metabolizable energy (ME) and significant effect (P<0.05) to decrease of methane and methane percentage. Supplementation IZ at a level of 10% was significantly higher for dry matter digestibility (DMD), organic matter digestibility (OMD), and microbial protein production (PPM) than diets treatment of R0, R2, and R3. The experiment concluded that Supplementation of I. zollingeriana (IZ) was able to reduce the methane gas production. Protein characteristics of IZ have easily degradable by rumen microbe showed the ammonia production was linearly increasing by 45.66% for each increasing level of IZ supplementation. Microbial protein production was higher (184.33 mg/ml) obtained of IZ supplementation up to 10% (R1). The experiment suggests doing protected protein of IZ when be used as a protein source in ruminant diets.
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Abdullah, L. 2014. Prospektif agronomi dan ekofisiologi indigofera zollingeriana sebagai tanaman penghasil hijauan pakan berkualitas tinggi. Pastura. 3 (2) : 79 – 83.
Abdullah, L., 2010. Herbage production and quality of shrub Indigofera treated by different concentration of foliar fertiliser. Med. Pet. 33 (3): 169–175.
Bach, A., S. Calsamiglia, and M. D. Stern. 2005. Nitrogen Metabolism in the Rumen. J. J. Dairy Sci. 88:(E. Suppl.):E9–E21.
Bioscreen Technologies. 2015. Bioscreen technologies research laboratories - artificial rumen by-pass analysis of rumen protected feed additives. https://www.youtube.com/watch?v=AAT63sytI0w.
Broderick, G.A. 2017. Optimizing ruminant conversion of feed protein to human food protein. Animal, 12 (8): 1722–1734
Cameron, K. C., H. J. Di, and J. L. Moir. 2013. Nitrogen losses from the soil/plant system: A review. Ann. Appl. Biol. 162:145–173.
Cieslak, A., P. Zmora, A. Matkowski, I. Nawrot-Hadzik, E. Pers-Kamczyc, M. El-Sherbiny1, M. Bryszak1 and M. Szumacher-Strabel. (2016). Tannins from Sanguisorba officinalis affect in vitro rumen Methane production and fermentation. The J. of Anim. & Plant Sci. 26(1): 54-62.
Denek, N., S.S. Aydin, A. Can. 2017. The effects of dried pistachio (Pistachio vera L.) by-product addition on corn silage fermentation and in vitro methane production. J Appl Anim Res. 45:185–189.
Fievez. V., O.J. Babayemi and D. Demeyer, D. 2005. Estimation of direct and indirect gas production in syringe: a tool estimate short chain fatty acid production the requires minimal laboratory facilities. Animal Feed Science and Technology. 5(1):197-210.
Franzolin, R., dan B.A. Dehority. 2010. The role of pH on the survival of rumen protozoa in steers. R. Bras. Zootec., 39 (10): 2262-2267.
General Laboratory Procedure. 1966. Report of Dairy Science. University of Wisconsin Madison.USA.
Getachew G., W. Pittroff, D.H. Putnam, A. Dandekar, S. Goyal, EJ. DePeters. 2008. The influence of addition of gallic acid, tannic acid or quebracho tannins to alfalfa hay on in vitro rumen fermentation and microbial protein synthesis. Anim Feed Sci Technol. 140:444–461.
Hariadi, B.T. and Santoso, B. 2010. Evaluation of tropical plants containing tannin on in vitro methanogenesis and fermentation parameters using rumen fluid. J. Sci. Food and Agric. 90 (3): 456-461.
Harun, A.Y. dan K. Sali. 2019. Factors affecting rumen microbial protein synthesis: A review. Vet Med Open J. 4(1): 27-35. Ružić-Muslić, D., M.P. Petrović, M.M. Petrović, Z. Bijelić, V. Caro-Petrović, N. Maksimović, V. Mandić. 2014. Protein source in diets for ruminant nutrition. J. Biotechnology in Animal Husbandry 30 (2), p 175-184.
Hristov, A. N. 2011. Contribution of ammonia emitted from livestock to atmospheric PM2.5 in the United States. J. Dairy Sci. 94:3130–3136.
Hristov, A. N., M. Hanigan, A. Cole, R. Todd, T. A. McAllister, P. M. Ndegwa, and A. Rotz. 2011. Ammonia emissions from dairy farms and beef feedlots: A review. Can. J. Anim. Sci. 91:1–35.
Kulling, D. R., H. Menzi, T. F. Krober, A. Neftel, F. Sutter, P. Lischer, and M. Kreuzer. 2003. Ammonia, nitrous oxide and methane emissions from differently stored dairy manure derived from grass- and hay-based rations. Nutrient Cycling in Agroecosystems 65: 13–22,
Kumalasari, N.R., G. P. Wicaksono, & L. Abdullah. 2017. Plant growth pattern, forage yield, and quality of Indigofera zollingeriana influenced by row spacing. Media Peternakan. 40(1):14-19.
Makkar, H.P.S. 2003. Effects and fate of tannins Ružić-Muslić, D., M.P. Petrović, M.M. Petrović, Z. Bijelić, V. Caro-Petrović, N. Maksimović, V. Mandić. 2014. Protein source in diets for ruminant nutrition. J. Biotechnology in Animal Husbandry 30 (2), p 175-184.
Menke, K.H., Steingass, H., 1988. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Anim. Res. Dev. 28, 7–55.
Mueller-Harvey, I., 2006. Unravelling the conundrum of tannins in animal nutrition and health. Journal of the Science of Food and Agriculture 86, 2010-2037.
Pathak, A.K. 2008. Various factors affecting microbial protein synthesis in the rumen. Review. Veterinary World, Vol.1(6): 186- 189
Penner G.B., J.R. Aschenbach, G. Gäbel, R. Rackwitz, and M.Oba. 2009. Epithelial capacity for apical uptake of short chain fatty acids is a key determinant for intraruminal pH and the susceptibility to subacute ruminal acidosis in sheep. J. Nutr. 139 (9), 1714-1720.
Russell J.B., J.D. O’Connor, D.G. Fox, P.J. Van Soest and C.J. Sniffen, 1992. A net carbohydrate and protein system for evaluating cattle diets. I. Ruminal fermentation. J Anim Sci 70:3551–3561.
Ružić-Muslić, D., M.P. Petrović, M.M. Petrović, Z. Bijelić, V. Caro-Petrović, N. Maksimović, V. Mandić. 2014. Protein source in diets for ruminant nutrition. J. Biotechnology in Animal Husbandry 30 (2), p 175-184.
Satter, L.D. dan L. L. Slyter. 1974. Effect of ammonia concentration on rumen microbial protein production in vitro. Brit. J. Nutr. 32:199-208.
Tilley, J.M.A. and R..A. Terry. 1963. A two-stage technique for the in vitro digestion of forage crops. Grass Forage Sci. 18:104-111.
Van Soest, P.J.1994. Nutritional Ecology of the Rumen (2nd Ed). Comstock Publishing Associates, Ithaca, NY. p 476.
DOI: http://dx.doi.org/10.33087/jiubj.v21i3.1736
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